Upon successful completion of this program the graduate will be able to:
1. Perform electron gun and full column alignment on both the SEM and TEM, to include critical objective astigmatism correction.
2. Identify SEM variables required for various sample and signal generation/collection situations and adjust accordingly.
3. Perform basic vacuum system startup, valving, shutdown, and maintenance.
4. Prepare an IC using Focused Ion Beam, tripod and planar polishing techniques for TEM and SEM.
5. Prepare required solutions and perform fixation, dehydration, and embedment or CPD/FF of various samples for TEM or SEM analysis.
6. Obtain artifact-free (knife marks, chatter, and/or compression) thick (0.5 um) and thin (100 nm) sections of mammalian, botanical and materials samples for TEM analysis.
7. Index single crystal and polycrystalline electron diffraction patterns for identification of crystal lattice orientation and compound identification.
8. Identify the structure and basic function of cellular organelles in mammalian tissues.
9. Perform electron gun filament change, aperture cleaning, and basic electronic troubleshooting to maintain equipment found in electron microscope labs.
10. Develop work habits such as timeliness, attendance, interpersonal skills, and communication skills consistent with employment requirements.
11. Be familiar with basic metallurgical principals such as phase diagrams, annealing, diffusion, dislocations, and twinning.
12. Evaluate sample and select proper SEM variables to collect representative x-ray spectra for accurate qualitative and standardless quantitative analysis.
Submitted 2/10/04 by Michael Kostrna